Catalogue of Molecular Biology Programs
                             Release 6.1
                            23 Jul 1999

All thanks are in the file biocatal.thanks.

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AC             BC00536
NAME           Anemona
DOMAIN         Metabolic regulation
DOMAIN         Enzyme kinetics
DESCRIPTION    ANEMONA.XLT is an EXCEL template for
DESCRIPTION    calculation of enzyme kinetic parameters by non-linear
DESCRIPTION    regression techniques. The tool is accurate as well as easy
DESCRIPTION    to use and modify.
DESCRIPTION    The different worksheets each deal with a
DESCRIPTION    different mathematical model. These sheets can be filled,
DESCRIPTION    used and saved in the same file or they can be copied and
DESCRIPTION    pasted into other EXCEL files, where they will retain the
DESCRIPTION    same properties as in the original.
DESCRIPTION    The models implemented are those
DESCRIPTION    corresponding to mono- or pseudo-monosubstrate reactions
DESCRIPTION    and the most common bisubstrate reactions:
DESCRIPTION    Michaelis-Menten, Double Michaelis-Menten, Hill model,
DESCRIPTION    Substrate Inhibition, the linear cases of reversible
DESCRIPTION    inhibition
DESCRIPTION    (Competitive, Incompetitive, Non-Competitive
DESCRIPTION    and Mixed Competitive-Incompetitive inhibition) and
DESCRIPTION    Ping-Pong, Ordered and Random bisubstrate mechanisms. Also,
DESCRIPTION    a weighted linear model has been implemented.
DESCRIPTION    If convergence is reached, the estimated
DESCRIPTION    values of the kinetic parameters are displayed, together
DESCRIPTION    with their standard error, covariances, final value of the
DESCRIPTION    Y function, the variance of fit and a X2-test of the fit.
DESCRIPTION    At the same time, graphs showing the
DESCRIPTION    original data and the fitted function versus
DESCRIPTION    the independent variable (concentration of substrate or
DESCRIPTION    inhibitor) and the residuals versus the independent
DESCRIPTION    variable are also provided. To aid in analysis of
DESCRIPTION    residuals, confidence limits for the residuals are also
DESCRIPTION    plotted.
AUTHOR         Hernandez, A., Ruiz, M.T.
RA             Hernandez, A. & Ruiz, M.T.
RT             "An EXCEL template for calculation of enzyme kinetic parameters
RT             by non-linear regression.";
RL             Bioinformatics 14(2):227-228(1998).
ADDRESS        Katholieke Universiteit Leuven
ADDRESS        Instituut voor Plantkunde
ADDRESS        Laboratorium voor Moleculaire Celbiologie
ADDRESS        Kardinaal Mercierlaan 92
ADDRESS        Heverlee
ADDRESS        Belgium B-3001
CONTACT        agustin.hernandez@bio.kuleuven.ac.be
SITE           ftp anonymous ftp.cc.kuleuven.ac.be
SITE           Directory /pub/science/
SITE-CONTACT   -
OS             Windows, Apple-McIntosh
LANGUAGE       EXCEL worksheet template
VOLUME         -
REQUIRES       EXCEL 5.0 or higher and SOLVER "Add-In"
COMMENTS       This version does not work in Spanish EXCEL due 
COMMENTS       to changes in the spelling of error messages.
COMMENTS       Users with other than English EXCEL may find
COMMENTS       difficulties for the same reason.

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